Journal: eLife
Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
doi: 10.7554/eLife.97373
Figure Lengend Snippet: ( A ) TAK1 overexpression activates p-IKK, p-JNK, p-ERK, p-P38 MAPK. ECA-109 cells were transfected with a plasmid expressing TAK1. 24 hr post-transfection, cells were collected for western blot analysis. ( B ) Quantification data for the blots in ( A ). ( C ) TAK1 knockdown represses p-IKK, p-JNK, p-ERK, p-P38 MAPK. ECA-109 cells were transfected with Map3k7 siRNA. 48 hr post-transfection, cells were harvested for western blot analysis. ( D ) Quantification data for the blots in ( C ). EV: empty vector; NC: negative control. Actin was used a loading control. *p < 0.05, **p < 0.01, and ***p < 0.001, by unpaired Student’s t-test. Figure 4—figure supplement 2—source data 1. Effects of TAK1 on p-IKK, p-JNK, p-ERK, and p-P38 MAPK in ECA-109 cells. Figure 4—figure supplement 2—source data 2. PDF file containing original western blots for , indicating the relevant bands. Figure 4—figure supplement 2—source data 3. Original files for western blot analysis displayed in .
Article Snippet: Rabbit monoclonal antibodies against TAK1 (#5206), phospho-TAK1 (Ser412, #9339), PKCα (#2056), phospho-PKC (pan) (gamma Thr514) (#38938), GSK-3β (#9315), phospho-GSK-3β (Ser9) (#5558), β-Catenin (#8480), phospho-β-Catenin (Ser33/37/Thr41) (#9561), MMP-2 (#87809), mouse monoclonal antibodies against Actin (#3700), Myc-Tag (Sepharose Bead Conjugate) (#55464), Epithelial-Mesenchymal Transition (EMT) antibody sample kit (#9782), phosphor-IKK (#2078), IKK (#61294), phosphor-JNK (#4668), JNK (#9252), phospho-ERK (#4370), ERK (#9102), phospho-P38 MAPK (#9211), P38 MPAK (#9212), and normal rabbit IgG (#2729) were from Cell Signaling Technology (Beverley, MA, USA).
Techniques: Over Expression, Transfection, Plasmid Preparation, Expressing, Western Blot, Knockdown, Negative Control, Control