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rabbit anti-pikk alpha(s176)/ikk-beta (s177)  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti-pikk alpha(s176)/ikk-beta (s177)
    Rabbit Anti Pikk Alpha(S176)/Ikk Beta (S177), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti-pikk alpha(s176)/ikk-beta (s177)/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    rabbit anti-pikk alpha(s176)/ikk-beta (s177) - by Bioz Stars, 2026-03
    90/100 stars

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    ( A ) TAK1 overexpression activates <t>p-IKK,</t> <t>p-JNK,</t> p-ERK, p-P38 MAPK. ECA-109 cells were transfected with a plasmid expressing TAK1. 24 hr post-transfection, cells were collected for western blot analysis. ( B ) Quantification data for the blots in ( A ). ( C ) TAK1 knockdown represses p-IKK, p-JNK, p-ERK, p-P38 MAPK. ECA-109 cells were transfected with Map3k7 siRNA. 48 hr post-transfection, cells were harvested for western blot analysis. ( D ) Quantification data for the blots in ( C ). EV: empty vector; NC: negative control. Actin was used a loading control. *p < 0.05, **p < 0.01, and ***p < 0.001, by unpaired Student’s t-test. Figure 4—figure supplement 2—source data 1. Effects of TAK1 on p-IKK, p-JNK, p-ERK, and p-P38 MAPK in ECA-109 cells. Figure 4—figure supplement 2—source data 2. PDF file containing original western blots for , indicating the relevant bands. Figure 4—figure supplement 2—source data 3. Original files for western blot analysis displayed in .
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    ( A ) TAK1 overexpression activates <t>p-IKK,</t> <t>p-JNK,</t> p-ERK, p-P38 MAPK. ECA-109 cells were transfected with a plasmid expressing TAK1. 24 hr post-transfection, cells were collected for western blot analysis. ( B ) Quantification data for the blots in ( A ). ( C ) TAK1 knockdown represses p-IKK, p-JNK, p-ERK, p-P38 MAPK. ECA-109 cells were transfected with Map3k7 siRNA. 48 hr post-transfection, cells were harvested for western blot analysis. ( D ) Quantification data for the blots in ( C ). EV: empty vector; NC: negative control. Actin was used a loading control. *p < 0.05, **p < 0.01, and ***p < 0.001, by unpaired Student’s t-test. Figure 4—figure supplement 2—source data 1. Effects of TAK1 on p-IKK, p-JNK, p-ERK, and p-P38 MAPK in ECA-109 cells. Figure 4—figure supplement 2—source data 2. PDF file containing original western blots for , indicating the relevant bands. Figure 4—figure supplement 2—source data 3. Original files for western blot analysis displayed in .
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    ( A ) TAK1 overexpression activates <t>p-IKK,</t> <t>p-JNK,</t> p-ERK, p-P38 MAPK. ECA-109 cells were transfected with a plasmid expressing TAK1. 24 hr post-transfection, cells were collected for western blot analysis. ( B ) Quantification data for the blots in ( A ). ( C ) TAK1 knockdown represses p-IKK, p-JNK, p-ERK, p-P38 MAPK. ECA-109 cells were transfected with Map3k7 siRNA. 48 hr post-transfection, cells were harvested for western blot analysis. ( D ) Quantification data for the blots in ( C ). EV: empty vector; NC: negative control. Actin was used a loading control. *p < 0.05, **p < 0.01, and ***p < 0.001, by unpaired Student’s t-test. Figure 4—figure supplement 2—source data 1. Effects of TAK1 on p-IKK, p-JNK, p-ERK, and p-P38 MAPK in ECA-109 cells. Figure 4—figure supplement 2—source data 2. PDF file containing original western blots for , indicating the relevant bands. Figure 4—figure supplement 2—source data 3. Original files for western blot analysis displayed in .
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    ( A ) TAK1 overexpression activates <t>p-IKK,</t> <t>p-JNK,</t> p-ERK, p-P38 MAPK. ECA-109 cells were transfected with a plasmid expressing TAK1. 24 hr post-transfection, cells were collected for western blot analysis. ( B ) Quantification data for the blots in ( A ). ( C ) TAK1 knockdown represses p-IKK, p-JNK, p-ERK, p-P38 MAPK. ECA-109 cells were transfected with Map3k7 siRNA. 48 hr post-transfection, cells were harvested for western blot analysis. ( D ) Quantification data for the blots in ( C ). EV: empty vector; NC: negative control. Actin was used a loading control. *p < 0.05, **p < 0.01, and ***p < 0.001, by unpaired Student’s t-test. Figure 4—figure supplement 2—source data 1. Effects of TAK1 on p-IKK, p-JNK, p-ERK, and p-P38 MAPK in ECA-109 cells. Figure 4—figure supplement 2—source data 2. PDF file containing original western blots for , indicating the relevant bands. Figure 4—figure supplement 2—source data 3. Original files for western blot analysis displayed in .
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    Image Search Results


    ( A ) TAK1 overexpression activates p-IKK, p-JNK, p-ERK, p-P38 MAPK. ECA-109 cells were transfected with a plasmid expressing TAK1. 24 hr post-transfection, cells were collected for western blot analysis. ( B ) Quantification data for the blots in ( A ). ( C ) TAK1 knockdown represses p-IKK, p-JNK, p-ERK, p-P38 MAPK. ECA-109 cells were transfected with Map3k7 siRNA. 48 hr post-transfection, cells were harvested for western blot analysis. ( D ) Quantification data for the blots in ( C ). EV: empty vector; NC: negative control. Actin was used a loading control. *p < 0.05, **p < 0.01, and ***p < 0.001, by unpaired Student’s t-test. Figure 4—figure supplement 2—source data 1. Effects of TAK1 on p-IKK, p-JNK, p-ERK, and p-P38 MAPK in ECA-109 cells. Figure 4—figure supplement 2—source data 2. PDF file containing original western blots for , indicating the relevant bands. Figure 4—figure supplement 2—source data 3. Original files for western blot analysis displayed in .

    Journal: eLife

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis

    doi: 10.7554/eLife.97373

    Figure Lengend Snippet: ( A ) TAK1 overexpression activates p-IKK, p-JNK, p-ERK, p-P38 MAPK. ECA-109 cells were transfected with a plasmid expressing TAK1. 24 hr post-transfection, cells were collected for western blot analysis. ( B ) Quantification data for the blots in ( A ). ( C ) TAK1 knockdown represses p-IKK, p-JNK, p-ERK, p-P38 MAPK. ECA-109 cells were transfected with Map3k7 siRNA. 48 hr post-transfection, cells were harvested for western blot analysis. ( D ) Quantification data for the blots in ( C ). EV: empty vector; NC: negative control. Actin was used a loading control. *p < 0.05, **p < 0.01, and ***p < 0.001, by unpaired Student’s t-test. Figure 4—figure supplement 2—source data 1. Effects of TAK1 on p-IKK, p-JNK, p-ERK, and p-P38 MAPK in ECA-109 cells. Figure 4—figure supplement 2—source data 2. PDF file containing original western blots for , indicating the relevant bands. Figure 4—figure supplement 2—source data 3. Original files for western blot analysis displayed in .

    Article Snippet: Rabbit monoclonal antibodies against TAK1 (#5206), phospho-TAK1 (Ser412, #9339), PKCα (#2056), phospho-PKC (pan) (gamma Thr514) (#38938), GSK-3β (#9315), phospho-GSK-3β (Ser9) (#5558), β-Catenin (#8480), phospho-β-Catenin (Ser33/37/Thr41) (#9561), MMP-2 (#87809), mouse monoclonal antibodies against Actin (#3700), Myc-Tag (Sepharose Bead Conjugate) (#55464), Epithelial-Mesenchymal Transition (EMT) antibody sample kit (#9782), phosphor-IKK (#2078), IKK (#61294), phosphor-JNK (#4668), JNK (#9252), phospho-ERK (#4370), ERK (#9102), phospho-P38 MAPK (#9211), P38 MPAK (#9212), and normal rabbit IgG (#2729) were from Cell Signaling Technology (Beverley, MA, USA).

    Techniques: Over Expression, Transfection, Plasmid Preparation, Expressing, Western Blot, Knockdown, Negative Control, Control

    Journal: eLife

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis

    doi: 10.7554/eLife.97373

    Figure Lengend Snippet:

    Article Snippet: Rabbit monoclonal antibodies against TAK1 (#5206), phospho-TAK1 (Ser412, #9339), PKCα (#2056), phospho-PKC (pan) (gamma Thr514) (#38938), GSK-3β (#9315), phospho-GSK-3β (Ser9) (#5558), β-Catenin (#8480), phospho-β-Catenin (Ser33/37/Thr41) (#9561), MMP-2 (#87809), mouse monoclonal antibodies against Actin (#3700), Myc-Tag (Sepharose Bead Conjugate) (#55464), Epithelial-Mesenchymal Transition (EMT) antibody sample kit (#9782), phosphor-IKK (#2078), IKK (#61294), phosphor-JNK (#4668), JNK (#9252), phospho-ERK (#4370), ERK (#9102), phospho-P38 MAPK (#9211), P38 MPAK (#9212), and normal rabbit IgG (#2729) were from Cell Signaling Technology (Beverley, MA, USA).

    Techniques: Transfection, Construct, shRNA, Recombinant, Plasmid Preparation, Sequencing, Activity Assay, Enzyme-linked Immunosorbent Assay, Software